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Laboratory exercises in microbiology

14,70 

ISBN: 978-83-7348-244-9

Wydanie: 1

Rok publikacji: 2008

Stron: 106

Opis

Laboratory exercises in microbiology

Holec-Gąsior Lucyna Kur JózefWerbowy Katarzyna

Wydawnictwo Politechniki Gdańskiej

 

 

Słowa kluczowe: enumeration of bacteria, antimicrobial agents, metabolic activities of microorganisms, microscopic techniques and bacteria staining, environmental protection and management, identification of unknown bacteria from environmental, cultivation of microorganisms, microbial relationships, environmental factors affecting microbial growth, sterilization and disinfection

 

„Laboratory Exercises in Microbiology” is a microbiology textbook written particularly for the students of the Environmental Protection & Management course. This edition was designed to provide an engaging and easy to understand textbook that is appropriate for a diverse student audience with varying backgrounds and science knowledge and helping students learn fundamental microbiology concepts without being over-whelmed by excessive detail. Each chapter begins with short learning objectives presenting key concepts that students should understand after studying each chapter. The following section of each chapter contains exercises suitable for the introductory course of microbiology. A laboratory report at the end of each chapter present students with questions that encourage them to apply what they have already learned.

Regardless of your reasons for approach to studying microbiology, enjoy yourself.

 

 

Contents

PREFACE

  1. STERILIZATION AND DISINFECTION

1.1. Physical sterilization

1.2. Chemical agents

  1. CULTIVATION OF MICROORGANISMS

2.1. Growth curve

2.2. Culture media

2.3. Nutritional requirements of cells

2.4. Microorganisms in the laboratory environment

2.4.1. Colony morphology

2.4.2. Growth patterns in broth, agar slant and agar deep-tube

2.5. Pure culture techniques

2.5.1. Spread plate technique

2.5.2. Streak plate technique

2.6. Specific media

  1. ENVIRONMENTAL FACTORS AFFECTING MICROBIAL GROWTH

3.1. Temperature

3.1.1. Effects on growth

3.1.2. Lethal effects

3.2. pH

3.3. Osmotic pressure

3.4. Ultraviolet light – lethal effects

3.5. Oxygen requirements – cultivation of anaerobes

  1. ENUMERATION OF BACTERIA
  2. ANTIMICROBIAL AGENTS
  3. METABOLIC ACTIVITES OF MICROORGANISMS

6.1. Hydrolysis of starch

6.2. Hydrolysis of fats

6.3. Hydrolysis of proteins

6.4. Casein hydrolysis

6.5. Fermentation and β-galactosidase activity

  1. MICROBIAL RELATIONSHIPS

07.1. Bacterial commensalism

07.2. Bacterial synergism

07.3. Microbial antagonism

  1. MICROSCOPIC TECHNIQUES AND BACTERIA STAINING

08.1. Microscopy

08.2. Microscope slide technique – bacteria staining

08.3. Gram staining

  1. IDENTIFICATION OF UNKNOWN BACTERIA FROM ENVIRONMENTAL SAMPLES
  2. MICROBIAL GENETICS

10.1. Izolation of plasmid DNA

10.2. Gel electrophoresis

10.3. Bacterial transformation

REFERENCES

 

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