Opis
Laboratory exercises in microbiology
Holec-Gąsior Lucyna, Kur Józef, Werbowy Katarzyna
Wydawnictwo Politechniki Gdańskiej
Słowa kluczowe: enumeration of bacteria, antimicrobial agents, metabolic activities of microorganisms, microscopic techniques and bacteria staining, environmental protection and management, identification of unknown bacteria from environmental, cultivation of microorganisms, microbial relationships, environmental factors affecting microbial growth, sterilization and disinfection
„Laboratory Exercises in Microbiology” is a microbiology textbook written particularly for the students of the Environmental Protection & Management course. This edition was designed to provide an engaging and easy to understand textbook that is appropriate for a diverse student audience with varying backgrounds and science knowledge and helping students learn fundamental microbiology concepts without being over-whelmed by excessive detail. Each chapter begins with short learning objectives presenting key concepts that students should understand after studying each chapter. The following section of each chapter contains exercises suitable for the introductory course of microbiology. A laboratory report at the end of each chapter present students with questions that encourage them to apply what they have already learned.
Regardless of your reasons for approach to studying microbiology, enjoy yourself.
Contents
PREFACE
- STERILIZATION AND DISINFECTION
1.1. Physical sterilization
1.2. Chemical agents
- CULTIVATION OF MICROORGANISMS
2.1. Growth curve
2.2. Culture media
2.3. Nutritional requirements of cells
2.4. Microorganisms in the laboratory environment
2.4.1. Colony morphology
2.4.2. Growth patterns in broth, agar slant and agar deep-tube
2.5. Pure culture techniques
2.5.1. Spread plate technique
2.5.2. Streak plate technique
2.6. Specific media
- ENVIRONMENTAL FACTORS AFFECTING MICROBIAL GROWTH
3.1. Temperature
3.1.1. Effects on growth
3.1.2. Lethal effects
3.2. pH
3.3. Osmotic pressure
3.4. Ultraviolet light – lethal effects
3.5. Oxygen requirements – cultivation of anaerobes
- ENUMERATION OF BACTERIA
- ANTIMICROBIAL AGENTS
- METABOLIC ACTIVITES OF MICROORGANISMS
6.1. Hydrolysis of starch
6.2. Hydrolysis of fats
6.3. Hydrolysis of proteins
6.4. Casein hydrolysis
6.5. Fermentation and β-galactosidase activity
- MICROBIAL RELATIONSHIPS
07.1. Bacterial commensalism
07.2. Bacterial synergism
07.3. Microbial antagonism
- MICROSCOPIC TECHNIQUES AND BACTERIA STAINING
08.1. Microscopy
08.2. Microscope slide technique – bacteria staining
08.3. Gram staining
- IDENTIFICATION OF UNKNOWN BACTERIA FROM ENVIRONMENTAL SAMPLES
- MICROBIAL GENETICS
10.1. Izolation of plasmid DNA
10.2. Gel electrophoresis
10.3. Bacterial transformation
REFERENCES
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